陳德喜的個(gè)人首頁(yè)

　　陳德喜，男，博士(后)，教授，博士生導(dǎo)師。北京市肝病研究所常務(wù)副所長(zhǎng)、首都醫(yī)科大學(xué)附屬北京佑安醫(yī)院感染中心副主任兼任艾滋病實(shí)驗(yàn)室主任。北京市衛(wèi)生系統(tǒng)“十百千”人才工程“十”層次人才。HIV相關(guān)神經(jīng)損傷及藥物毒性研究方向的學(xué)科帶頭人。1963年9月生，山東省人。1997年北京醫(yī)科大學(xué)生物化學(xué)與分子生物學(xué)專(zhuān)業(yè)博士畢業(yè)后到美國(guó)匹茲堡大學(xué)醫(yī)學(xué)院神經(jīng)生物學(xué)系做博士后工作。2000 年到2006 年在美國(guó)俄勒岡州立醫(yī)科大學(xué)做助理教授，研究工作涉及生物化學(xué)與分子生物學(xué)多個(gè)領(lǐng)域，主要從事神經(jīng)生物學(xué)、細(xì)胞凋亡等研究工作。2006 年被首都醫(yī)科大學(xué)附屬北京佑安醫(yī)院引進(jìn)，籌建新的性病艾滋病實(shí)驗(yàn)室并先后任副主任、主任、北京市肝病研究所副所長(zhǎng)、常務(wù)副所長(zhǎng)。

　　基金項(xiàng)目

　　1.The construction of gp120 transgenic mice by ASPP2/P53 knockout mice used to investigate the pathogenesis of HIV-1 associated dementia. NSFC: 30770742, 2008-2010. 360,000RMB

　　2.The role of ASPP2 and p53 in HIV-1 associated dementia induced by gp120. NSFC: 30870853, 2009-2011. 340,000RMB

　　3.The virological characteristics and neurotoxicity mechanism of HIV-1 B’ subtype in the central nervous system. NSFC: 30910103915, 2010-2013. 1 million RMB

　　4.The correlation study of chomekine CCL3L1 and HIV-1 Susceptibility. Beijing Natural Science Foundation: 7092045. 2009-2011,200,000 RMB

　　5.The role of HIV infection and immune in the central nervous system in the pathogenesis of AIDS. Beijing Natural Science Foundation: 7101005. 2010-2013, 500,000RMB

　　6.項(xiàng)目名稱(chēng)：我國(guó)耐藥HIV 毒株發(fā)生和傳播的監(jiān)控以及監(jiān)測(cè)預(yù)警系統(tǒng)的建立

　　項(xiàng)目類(lèi)別：國(guó)家十二五重大專(zhuān)項(xiàng)(2008ZX10102)(自課題負(fù)責(zé)人)

　　起止年月：2012.1-2015.12， 資助金額：人民幣110萬(wàn)元

　　7. 項(xiàng)目名稱(chēng)：趨化性細(xì)胞因子CCL3L1與HIV-1病理進(jìn)程的研究

　　項(xiàng)目類(lèi)別：北京市教委項(xiàng)目(第1負(fù)責(zé)人)

　　起止年月：2009.1-2011.12， 資助金額：人民幣14萬(wàn)元

　　8. 項(xiàng)目類(lèi)別：人事部歸國(guó)人員優(yōu)先專(zhuān)項(xiàng)資助基金(第1負(fù)責(zé)人)

　　起止年月：2009.1-2010.12，資助金額：人民幣7萬(wàn)元

　　9. 項(xiàng)目名稱(chēng)： ASPP2和角蛋白18對(duì)HCV所至肝損傷的影響

　　項(xiàng)目類(lèi)別：北京市醫(yī)學(xué)發(fā)展基金(第1負(fù)責(zé)人)

　　起止年月：2008.1-2010.12， 資助金額：人民幣15萬(wàn)元

　　10. 項(xiàng)目名稱(chēng)：我國(guó)耐藥HIV 毒株發(fā)生和傳播的監(jiān)控以及監(jiān)測(cè)預(yù)警系統(tǒng)的建立

　　項(xiàng)目類(lèi)別：國(guó)家十一五重大專(zhuān)項(xiàng)(2008ZX10102)(自課題負(fù)責(zé)人)

　　起止年月：2008.10-2010.12， 資助金額：人民幣190萬(wàn)元

　　11. 項(xiàng)目名稱(chēng)：中國(guó)艾滋病病人的抗病毒治療和免疫重建研究

　　項(xiàng)目類(lèi)別：國(guó)家十一五重大專(zhuān)項(xiàng)(2008ZX10103)(自課題負(fù)責(zé)人)

　　起止年月：2008.10-2010.12，資助金額：人民幣100萬(wàn)元

　　12. 項(xiàng)目名稱(chēng): 北京市艾滋病防控策略研究項(xiàng)目：

　　子課題：艾滋病高危人群監(jiān)測(cè)、篩查及防空策略研究(課題第2負(fù)責(zé)人)

　　項(xiàng)目類(lèi)別：北京市科委主題計(jì)劃項(xiàng)目：(D0906003040591)

　　研究起止年月: 2006.1-2008.12， 獲資助金額：人民幣100萬(wàn)元

　　13. 項(xiàng)目名稱(chēng):實(shí)用快速HIV-1抗藥表型篩選及其臨床意義(第1負(fù)責(zé)人)

　　項(xiàng)目類(lèi)別：北京市豐臺(tái)區(qū)科技項(xiàng)目(第1負(fù)責(zé)人)

　　研究起止年月: 2007.6-2008.6。 獲資助金額：人民幣10萬(wàn)元

　　14. 項(xiàng)目名稱(chēng): Behavioral Interventions for Men who have Sex with Men

　　項(xiàng)目類(lèi)別：美國(guó)China AIDS Fund基金(第1負(fù)責(zé)人)

　　研究起止年月: 2008.1-2009.1， 獲資助金額：美元2萬(wàn)元

　　15. 北京市“十， 百，千”的“十”類(lèi)人才;資助 20萬(wàn)(含院內(nèi)配套10萬(wàn))。

　　16. 北京市衛(wèi)生局學(xué)科帶頭人;資助90萬(wàn)，院內(nèi)配套90萬(wàn)元。

　　發(fā)明專(zhuān)利

　　The method of detection base excision repair by real-time quantitative PCR (201010195750.0).

　　The construction of TA eukaryotic expression vector traced by secreted alkaline phosphatase (201010107709.3).

　　The construction of the vector used for detection HIV-1 phenotypic drug resistance (201110053354.9).

　　The monoclonal antibody production and application of intercellular adhesion molecule 5 (201210051653.3).

　　獲獎(jiǎng)情況

　　趨化性細(xì)胞因子CCL3L1與HIV-1易感性和融合蛋白與多克隆抗體的研制榮獲2007年度豐臺(tái)區(qū)科學(xué)技術(shù)獎(jiǎng)三等獎(jiǎng)(第一完成人)。2011年，獲評(píng)北京市優(yōu)秀人才。2012年，獲第四屆北京市留學(xué)人員創(chuàng)新創(chuàng)業(yè)特別貢獻(xiàn)獎(jiǎng)。

　　學(xué)術(shù)組織

　　Society for Neuroscience 1998-present

　　American Association for Cancer Research 2003 – present

　　臨床肝膽病雜志 編委

　　職稱(chēng)：教授，博士生導(dǎo)師

　　科室：感染中心

　　專(zhuān)業(yè)擅長(zhǎng)：

　　肝病與艾滋病等感染與傳染性疾病的基礎(chǔ)與臨床基礎(chǔ)和綜合防治研究。

　　論文：

　　1.Li Li1,2#, Ronghua Jin2#, Xiaoli Zhang3, Fudong Lv 2, Lifeng Liu 1, Daojie Liu1, Kai Liu1, Ning Li2*, Dexi Chen1,2*. Oncogenic activation of GPC3 by c-Myc in human hepatocellular carcinoma. Hepatology, 2012(Accepted).

　　2.Zhang Y, Wang M, Ding W, Wei F, Zhao Q, Wang X, Shi Y, Li N, Chen D. Accumulation of nuclear and mitochondrial DNA damage in the frontal cortex cells of patients with HIV-associated neurocognitive disorders. Brain Res. 2012 [Epub ahead of print]

　　3.Liu Z, Li L, Chen Z, Xu M, Zhang T, Jiao Y, Sheng B, Chen D*, Wu H*. Prevalence of GB virus type C viraemia in MSM with or without HIV-1 infection in Beijing, China. Epidemiol Infect. 2012 Mar 26:1-11.

　　4.Zhang Y, Qiao L, Ding W, Wei F, Zhao Q, Wang X, Shi Y, Li N, Smith D, Chen D. An initial screening for HIV-associated neurocognitive disorders of HIV-1 infected patients in China. J Neurovirol. Volume 18, Issue 2 (2012), Page 120-126

　　5.Yulin Zhang, Ying Shi, Luxin Qiaob, et al. Sigma-1 receptor agonists provide neuroprotection against gp120 via a change in bcl-2 expression in mouse neuronal cultures Brain Res. 2012 Jan 11;1431:13-22.

　　6.Lifeng Liu, Qingxia Zhao, Feili Wei, Ning Li，YingShi， Dexi Chen* . Genetic Analysis of HIV Type 1 env Gene in Cerebrospinal Fluid and Plasma of Infected Chinese Paid Blood Donors. AIDS Research and Human Retroviruses。 AIDS Res Hum Retroviruses. 2011 Jul 19. [Epub ahead of print]

　　7.Daijun Xiang, Qing Xia, Dexi Chen, Feng Xia, Yan Zhao, Ning Li, Huiping Yan. Detection of autoantibodies to D-3-phosphoglycerate dehydrogenase in patients with chronic liver disorders and analysis of their clinical relevance. Hepatology research. 2011 Jun 28. [Epub ahead of print]

　　8.Xiaoli Zhang , Amy Farrell, Colin Daniel, Hugh Arnold, Charles Scanlan, Bryan Laraway , Mahnaz Janghorban , Lawrence Lum , Dexi Chen , Megan Troxell , Rosalie Sears . Mechanistic insight into c-Myc stabilization in breast cancer involving aberrant Axin1 expression. Proc Natl Acad Sci U S A. 2011 Aug 1. [Epub ahead of print]

　　9.Jin R, Sun Y, Qi X, Zhang H, Zhang Y, Li N, Ding W, Chen D E2F1 is Involved in DNA Single-strand Break Repair through Upregulation of Cell-cycle-dependent XRCC1 Expression. DNA repair。 DNA Repair (Amst). 2011 Jul 12. [Epub ahead of print]

　　10.Hua W, Jiao Y, Zhang H, Zhang T, Chen D, Zhang Y, Chen X, Wu H.Central Memory CD4 Cells Are an Early Indicator of Immune Reconstitution in HIV/AIDS Patients with Anti-Retroviral Treatment. Immunol Invest. 2011 May 12. [Epub ahead of print]

　　11.Feili Wei#, Xicheng Wang#, Lifeng Liu, Rong Gao，Luxin Qiao, YingShi， Dexi Chen* Characterization of HIV Type 1 env Gene in Cerebrospinal Fluid and Blood of Infected Chinese Patients， AIDS Research and Human Retroviruses (Online Ahead of Print: March 27, 2011)

　　12.Zhang H, Zang Y, Sun Y, Jin R, Wu H, Wang M, Li N, Chen D#. A new approach utilizing real-time qPCR to detect in vitro base excision repair. DNA repair, 2010; 9(8):898-906.

　　13.Shi Y, Sun S, Liu Y, Li J, Zhang T, Wu H, Chen X, Chen D*, Zhou Y*. Keratin 18 phosphorylation as a progression marker of chronic hepatitis B. Virol J. 2010 Mar 24;7:70

　　14.Kampa, K.M.1,2, Acoba, J.D.2, Chen, D.2, Gay, J.2, Lee, H-J.2, Beemer, K.2, Padiernos, E.2, Boonmark, N.3, Zhu, Z.2, Fan, A.C.3,Baily, A.2, Flemming, W.H.2, Corless, C.2, Felsher, D.W.3, Naumovski, L.3,# and Lopez, C.D.2 Apoptosis Stimulating Protein of p53 (ASPP2) Heterozygous Mice Are Tumor Prone and Have Attenuated Cellular Damage-Response Thresholds.。 PNAS　2009 106(11):4390-5

　　15.Huang Chun#, Wu Hao#, Zhang Honghai, Li Ning, Wu Yasong, Dexi Chen*. CCl3L1 preventing gp120-induced neuron death via CREB cell signal pathway.. Brain Research 2009 1257:75-88..

　　16.Wu Yasong*, Li Ning, * Zhang Tong, Wu Hao, Huang Chun, Chen Dexi#. Mitochondrial DNA base excision repair and mitochondrial DNA mutation in human hepatic HuH-7 cells exposed to stavudine. Mutation Research 2009 12;664(1-2):28-38

　　17.Dexi Chen, Zhiyong Yu, Zhiyi Zhu, Lopez CD. E2F1 regulates the base excision repair gene XRCC1 and promotes DNA repair.. JBC 2008, 283 (22), 15381-9.

　　18.*Li N, Wu H, Yang S, Chen D†. Ischemic preconditioning induces XRCC1, DNA polymerase-beta, and DNA ligase III and correlates with enhanced base excision repair. DNA Repair, 2007, 6:1297-1306.

　　19.Dexi Chen , Zhiyi Zhu, Zhiyong Yu, Charles D. Lopez . An intact p53 pathway is required for efficient mitochondrial BER. Cancer Research. 2006,66(&):3485-3494.

　　20.Zhiyi Zhu, Jason Ramos, Kerstin Kampa, Zhiyong Yu, Dexi chen, Charles Lopez. ASPP2 Apoptotic is Modulated by Proteasomal Degradation. J. Biol. Chem. 2005 288(41): 34473-88.

　　21.Chen D, Padiernos E, Ding F, Lossos IS, Lopez CD. Apoptosis-stimulating protein of p53-2 (ASPP2/53BP2L) is an E2F target gene.Cell Death Differ. 2005 Apr;12(4):358-68

　　22.Meller R, Minami M, Cameron J, Chen D, Lan J, HenshaLL D, SIMON R. CREB-mediated Bcl-2 protein expression following ischemic preconditioning. 2005 Feb;25(2):234-46.

　　23.Dexi Chen, Manabu Minami, David C. Henshall; Roger P. Simon. Preconditioning Ischemia Enhances Base-Excision Repair Capability within Rat Brain Mitochondrial DNA. J Cereb Blood Flow Metab. 2003 Jan;23(1):88-98.

　　24.Dexi Chen‡, Kunlin Jin*, Kenji Kawaguchi†, Masaki Nakayama†, Xiaoman Zhou‡, Zhigang Xiong‡, An Zhou‡, David A. Greenberg*, Steven H. Graham†, and Roger P. Simon‡. Ero1-L, an Ischemia-Inducible Gene from Rat Brain with Homology to Global Ischemia-Induced Gene 11 (Giig11), is Localized to Neuronal Dendrites by a Dispersed Identifier (ID)Element-Dependent Mechanism. J Neurochem. 2003 May;85(3):670-9

　　25.Dexi Chen, Guodong Cao, Cristine O’Horo, Wei Pei and Jun Chen. DNA Base-Excision Repair Activity for Oxidative Lesions in Rat Brain Mitochondria: Marked Age-dependent Regulation. J Neurochem 2002 81 1273-1284.

　　26.K. Jin1, X.O. Mao1, M.C. Eshoo1, G. del Rio1, R. Rao1 , D. Chen2, R.P. Simon2 and D.A. Greenberg.1cDNA Microarray Analysis of Changes in Gene Expression Induced by Neuronal Hypoxia In Vitro. Neurochem Res. 2002 Oct;27(10):1105-12.

　　27.Xiangping Chu; J. Jefferson; M. Johnson; L. Root; X. Zhu.; Dexi Chen; R. Simon; Z. Xiong. PC12 cells contain a singtle population of functional protein-gated channels-homomultimeric asic1A. J Neuroph 2002, 87 2555-256.

　　28.Martha B. Johnson, Manabu Minami, Dexi Chen, Roger P. Simon. Global ischemia induces expression of Asic2 in rat brain. J Cereb Blood Flow Metab. 2001, 21:734-740

　　29.Li YY, Chen D, Watkins SC, Feldman AM..Mitochondrial Abnormalities in Tumor Necrosis Factor-alpha-Induced Heart Failure Are Associated With Impaired DNA Repair Activity. Circulation. 2001 Nov 13;104(20):2492-7

　　30.Cao G, Minami M, Pei W, Yan C, Chen D, O\'Horo C, Graham SH, Chen J. Intracellular Bax translocation after transient cerebral ischemia: implications for a role of the mitochondrial apoptotic signaling pathway in ischemic neuronal death.

　　31.J Cereb Blood Flow Metab. 2001 Apr;21(4):321-33.

　　32.Chen D, Stetler RA, Cao GD, Pei W, Yin X-M, Chen J. Characterization of the rat DFF35/ICADs: the endogenous inhibitor of DNA fragmentation in neuronal apoptosis. J Biol Chem. 2000 Dec 8;275(49):38508-17.

　　33.Chen D, Jing Lan, Wei Pei, Jun Chen. Detection of DNA Base-Excision repair activity for oxidative lesions in adult rat brain mitochondria. J Neurosci Res 2000. 61(2):225-236.

　　34.Yan C, Chen J, Chen D, Minami M, Pei W, Yin XM, Simon RP. Overexpression of the cell death suppressor Bcl-w in ischemic brain: implications for a neuroprotective role via the mitochondrial pathway. J Cereb Blood Flow Metab 2000 Mar;20(3):620-30.

　　35.Nagayama T, Lan J, Henshall DC, Chen D, O\'Horo C, Simon RP, Chen J.Induction of oxidative DNA damage in the peri-infarct region after permanent focal cerebral ischemiaJ Neurochem 2000 Oct;75(4):1716-28

　　36.Nagayama T, Simon RP, Chen D, Henshall DC, Pei W, Stetler RA, Chen J .Activation of poly(ADP-ribose) polymerase in the rat hippocampus may contribute to cellular recovery following sublethal transient global ischemia. J Neurochem 2000 Apr;74(4):1636-45.

　　另外，中文核心期刊以上文章100篇

　　學(xué)術(shù)交流：

　　1.Kathryn Van Hook*, Zhiping Wang, Hun Joo Lee, Zhiyi Zhu, Dexi Chen#, and Charles Lopez#. ΔN-ASPP2 is a novel isoform of the ASPP2 tumor suppressor and a regulator of ASPP2 function.102th AACR meeting, Aprl2-6 2011, Orlando， Florida， USA

　　2.Dexi Chen*, Fujie Zhang, Yu Sun, Ning Li, Sufang Yang, Yulin Zhang, Feili Wei, Luxin Qiao.The p53 isoforms ?133p53 and ?40p53 lead to a decrease in DNA polymerase y activity stimulated by wtp53 in both the presence and absence of d4T. Genomic Instability and DNA Repair (B4) January 30 - February 4, 2011 • Keystone Resort, Keystone, Co, USA

　　3.Ronghua Jin, Huiguo Ding, Dexi Chen, Ning Li.. E2F1 up-regulation of XRCC1 expression and contribution to DNA single-strand break repair in cell cycle. Presentation in AACR 100th annual meeting, 2009, April 22 – 28 Denver. USA.

　　4.Dexi Chen, Zhiyong Yu, Zhiyi Zhu, Lopez CD. E2F target to XRCC1and contribution to single strand broken repair in S phase. Presentation in AACR 97th annual meeting, 2006, April 1 – 5 Washington DC.

　　5.Huiguo Ding, Dexi Chen, Chunhui Zhao, Hongwei Shang, Li Zhou, Juan Zhang, Xingcui Wang, Xinyue Chen. Flap endonuclease-1 plays double roles in cisplatin-based chemoresistance and cell apoptosis resulted from enhanced long patch DNA repair and a gap endonuclease activity. Presentation in AACR 97th annual meeting, 2006, April 1 – 5 Washington DC

　　6.Dexi Chen , Zhiyi Zhu, Zhiyong Yu, Charles D. Lopez . An intact p53 pathway is required for efficient mitochondrial BER. Presentation in AACR 96th annual meeting, 2005 April 16 – 20 Los Angel.

　　7.Dexi Chen, Zhiyi Zhu, Emerson Padiernos, Izidore S. Lossos, Charles D. Lopez. ASPP2/53BP2 is an E2F target gene and many link the p53 and RB/E2F pathways. American Association for Cancer Research annual meeting (95) March 27-31, 2004 Orlando, Florida.

　　8.Dexi Chen, Manabu Minami, Martha B. Johnson, Jing Quan Lan, Roger P. Simon

　　9.The induction of Bcl-2 expression by CREB and CBP contributes to neuronal survival in focal ischemic tolerance. Society for neuroscience annual meeting (31). San Diago, CA. 2001

　　10.Dexi Chen, Guodong Cao, Cristine O’Horo, Wei Pei and Jun Chen. DNA Base-Excision Repair Activity for Oxidative Lesions in Rat Brain Mitochondria: Marked Age-dependent Regulation, Society for neuroscience annual meeting (30). New Orleans, Louisiana. Nov, 4-9, 2000.

　　11.Guodong , Cao Dexi Chen, Cristine O’Horo, Wei Pei , Yougjie Liu and Jun Chen. Cloning and characterization of rat Caspase-9: implication for neuronal cell death after transient cerebral ischemia and cerebellar development. Society for neuroscience annual meeting (30). New Orleans, Louisiana. Nov, 4-9, 2000.

　　12.Guodong Cao, Dexi Chen, Roger, P.S. and Jun Chen. Molecular cloning and characterization of rat caspasae-activated deoxyribonuclease(CAD): Implication for neuronal cell death after transient cerebral ischemia. Society for neuroscience annual meeting (29). Miami Beach, Florida. Oct, 23-28,1999.

　　13.Dexi Chen,W Pei, DA Greenberg, S Irvin, A sinor, SH Granam, J Chen et al. Necrosis in primary cultures of cortical neurons following ischemia-like insults: Induction of DNA strand breaks as a death mediator. Society for neuroscience annual meeting(28). Cos Angeles, Calif . Nov. 7-12, 1998.

　　14.Jing Lan, Dexi, Chen, M Minami, and J, Chen. Inducible DNA base excision repair capacity and the evolution of oxidative DNA damage in rat brain following transient cerebral ischemia. Society for neuroscience annual meeting (29). Miami Beach, Florida. Oct, 23-28,1999.

　　15.Y. Luo, J. Lan, G Cao, D. Chen, R.A. Steteler, C.H. Yan, G.Y. Yang, R.P. Simon, and J. Chen. Activation of caspase-activated deoxyribonuclease(CAD) mediates DNA fragmentation in mice following transient cerebral ischemia. Society for neuroscience annual meeting (29). Miami Beach, Florida. Oct, 23-28,1999.

　　16.R.A. Stetler, W. Pei, D. Chen, K. Tanaka, and J. Chen. Caspase-mediated neuronal cell death induced by mitochondrial complex I inhibitation by BCL-X-long overexpression. Society for neuroscience annual meeting (29). Miami Beach, Florida. Oct, 23-28,1999.